Simultaneous Determination of Calcium and Magnesium of Serum by a Single Chelometric Titration.
نویسندگان
چکیده
Titration of a trichloracetic acid filtrate of serum with EDTA is carried out at pH 9.8 using an excess of Calmagite indicator. Successive photometric readings of percentage transmission, made with an EEL titrator, are plotted against the volume of EDTA added. There is very little change at first while calcium is being complexed; the reading then decreases rapidly while magnesium is being titrated, followed by a flattening of the curve at completion of the titration. Hence, two sharp “breaks” are obtained in the titration curve, the first corresponding to the amount of calcium and the second to the amount of magnesium.
منابع مشابه
A New Indicator for the Chelometric Measurement of Calcium in Serum and Urine.
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متن کاملConcurrent determination of total serum calcium and magnesium by thermometric titration with ethylenediaminetetraacetate.
Total serum calcium and magnesium may be determined in one thermometric titration, with disodium ethylenediaminetetraacetate as the titrant. A 1-ml serum sample is diluted with 1 ml of tris(hydroxymethyl)aminomethane buffer (pH 8) and titrated at a constant rate with a motorized syringe buret. Results by the thermometric method compared well with those by atomic absorption spectroscopy.
متن کاملA simple and rapid method for the simulataneous determination of calcium and magnesium from the same sample of blood serum.
A simple and rapid procedure has been developed for the complexometric titration of serum calcium and magnesium using "plasmocorinth B" as indicator. Both determinations can be carried out from the same 0.5 ml. sample. The method is in good agreement with the established calcium and magnesium methods.
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ورودعنوان ژورنال:
- Clinical chemistry
دوره 10 شماره
صفحات -
تاریخ انتشار 1964